Ideal for ultrafilt ration, diafiltration and microfiltration, single-use hollow fiber filters for tangential flow
filtration are packed in housings engineered to handle high pressures, with a choice of fiber chemistries
and sizes from process development to manufacturing.
Hollow fiber filter modules for tangential flow filtration (TFF) from Repligen are industry standards in the filtration and concentration of biologics. Manufactured in compliance
with ISO guidelines, the filters are used in thousands of Krosflo TFF Sysytems and XCellTM ATF worldwide, as well as other industry hollow fiber TFF systems.
Hollow fiber filter TFF modules are offered with a broad range of sizes, surface areas and membrane chemistries designed to facilitate easy scale-up from small volume
process development and R&D applications to commercial production. During scale-up, adding more fibers to process larger volumes enables consistency in the length of the
filtration flow path even while the surface area of the filter is increased.
| Primary Application |
Volume | Inlet/ Outlet |
Side Port |
Scale Length |
Effective Length (㎝) |
Total Length (㎝) |
Surface Area |
|
|---|---|---|---|---|---|---|---|---|
| MicroKros | ||||||||
 |
Process Characterization,Small-volumeTFF in a laboratory |
1~100ml | MLL | FLL | 1ⅹFL 2ⅹFL 3ⅹFL |
20 41.5 65 |
25 45 69 |
13 - 20㎠ 26 - 59㎠ 41 - 92㎠ |
| MidiKros | ||||||||
 |
Process developmentat small scale |
100ml~3L | FLL | FLL | 1ⅹFL 2ⅹFL 3ⅹFL |
20 41.5 65 |
25 45 69 |
75- 190㎠ 155 - 390㎠ 245- 610㎠ |
| MidiKrosTC | ||||||||
 |
Process development atpilot scale |
100ml~3L | 1/2” TC | FLL | 1ⅹFL 2ⅹFL 3ⅹFL |
20 41.5 65 |
25 45 69 |
75- 190㎠ 155 - 390㎠ 245- 610㎠ |
| MiniKrosSampler | ||||||||
 |
Batch TFF of small volumes | 3L~15L | 0.75” TC | 0.75” TC | 1ⅹFL 2ⅹFL 3ⅹFL |
20 41.5 65 |
25 47 70 |
490- 1000㎠ 1000 - 2200㎠ 1600- 3400㎠ |
| MiniKros | ||||||||
 |
Pilot-scaleTFF | 5L~50L | 1.5” TC | 0.75” TC | 1ⅹFL 2ⅹFL 3ⅹFL |
20 41.5 65 |
25 46 70 |
0.16- 0.31㎡ 0.32 - 0.65㎡ 0.51- 1.0㎡ |
| KrosFlo® | ||||||||
 |
Production-scale TFF | 10L~100L | 3” TC | 1.5” TC | 1ⅹFL 2ⅹFL 3ⅹFL |
20 41.5 65 |
23 46 69 |
0.78- 1.6㎡ 1.65 - 3.25㎡ 2.55- 5.10㎡ |
| KrosFlo®MAX | ||||||||
 |
Production-scale TFF | 100L~ 1000+L |
6” TC | 1.5” TC | R65 3ⅹFL R85 |
49.9 67.9 107.5 |
73.5 91.5 131.1 |
4.30- 10.40㎡ 7.10 -17.10㎡ 11.30- 11.35㎡ |
| Membrane | Fiber internal diameter | MWCO and Pore Size | # Module Configurations | # Effective Lengths | |
|---|---|---|---|---|---|
| mPES | Modified Polyethersulfone | 0.50 mm | 3kD, 5kD, 10kD, 30kD, 50kD, 70kD,100kD, 300kD, 500kD, 750kD | 7 | 3 |
| mPES | Modified Polyethersulfone | 0.75 mm | 0.65µm | 7 | 3 |
| mPES | Modified Polyethersulfone | 1.00 mm | 3kD, 5kD, 10kD 30kD, 50kD, 70kD, 100kD, 300kD, 500kD, 750kD | 7 | 3 |
| ME | Mixed Cellulose Ester | 0.63 mm | 0.1µm, 0.2µm | 7 | 3 |
| ME | Mixed Cellulose Ester | 1.00 mm | 0.2µm | 7 | 3 |
| PS | Polysulfone | 0.5 mm | 10kD, 50kD, 500kD and 0.05µm | 7 | 3 |
| PES | Polyethersulfone | 0.5 mm | 0.2µm | 7 | 3 |
| PES | Polyethersulfone | 1.0 mm | 0.2µm | 7 | 3 |
| Applications | Membraneselection | mPES | ME | PS | PES |
|---|---|---|---|---|---|
| Concentration and diafiltration(buffer exchange) of proteins, peptides, enzymes, recombinant proteins | 1, 3, 5, 10, 30, 50, 70,100kD | x | x | ||
| Depyrogenation ofwater / buffers | 3, 10kD | x | x | ||
| Coating, diafiltration,fractionation and / or dewatering of nanoparticles | 70, 100, 300, 500, 750kD, 0.2µm | x | x | ||
| Concentration and diafiltration ofalbumin (nominal MW 67kd) | 10kD | x | |||
| Concentration and diafiltration ofmonoclonal antibodies (155kd) | 30kD | x | |||
| Concentration and diafiltration ofenvelope and non-envelope virus | 50,70, 100, 300, 500, 750kD, 0.05µm | x | x | ||
| Clarification of cell lysates,viral-expressed cultures to recover product in permeate | 500, 750kD, 0.05, 0.1µm | x | x | x | |
| Concentration and washing of insect,bacterial and/or mammalian cells | 750kD, 0.1, 0.2, 0.65µm | x | x | ||
| Continuous bioreactor perfusion of cellculture | 30, 50, 70, 100, 300, 500, 750kD, 0.2,0.65µm | x | x | x | |
| Concentration and diafiltration ofsingle-stranded or double-stranded nucleic acids | 1, 3, 5, 10, 30, 50, 70, 100kD | x | |||
| Washing latex, polystyrene and otherdiagnostic particles to remove un-reactives / unbound drug substance | 500, 750kD, 0.05, 0.1, 0.2, 0.65µm | x | x | x |
